Cours international

DNA Replication, Genetic instability and Cancer - 2012

The aim is to describe the current knowledges in the fields of DNA replication, repair and recombination and its consequences in genetic instability and cancer.

Key to the proper inheritance of the genetic information, cells should duplicate their genome before mitosis and avoid mutagenesis. The course will describe the mechanisms and the regulations that ensure the maintenance of genetic information across cell generations. Focus will be put on initiation and completion of replication along the bulk chromosomes and at specific regions such as the telomeres ; the pathways of DNA repair and recombination ; the relationships linking replication stress to the formation of DNA damages ; the signalling of intrinsic and environmentally induced DNA damages ; the interferences with the transcriptional activity ; the variety of cellular responses to damages including the generation of chromosome rearrangements and oncogenesis. A large set of experimental methods involving genetic, molecular, cellular, biochemical approaches will be taught.

Organizers: Michelle Debatisse, Franck Toledo, Alain Nicolas

Master 2 or PhD students from France and abroad.

In advance, a scientific article chosen on the theme of the course will be attributed to the M2 students who will present and discuss orally this article and their expanded bibliographical analysis in a 20 minutes oral presentation +15 min Discussion. This presentation can be evaluated by the organizers for University validation.

All participants should attend the 5 days course. Final acceptance of the PhD participants will require a signed engagement from their thesis supervisor attesting the student’s availability for the whole week. This engagement should be addressed upon application by Email at

This course is validated by UPMC - Paris VI University. It provides 3 ECTS or an equivalent of 30 hours.

The courses will be in English. The participants and speakers will meet every day during lunch time on site.

Selection of application files by the organization committee.

April 2 - 6, 2012
6 January 2012

Amphithéâtre Pierre et Marie Curie, 11 rue Pierre et Marie Curie, 75005 Paris, France

January 16th, 2012

No registration fees

Alain Nicolas
Franck Toledo
Michelle Debatisse
Arturo Londono-Vallejo
Bernard Malfoy
Antonin Morillon
Members of the Unit IC/CNRS UMR3244/UPMC

To provide an updated overview of the mechanisms and the regulation of the replication repair and recombination processes, the sources and the signalling of the damages, the cellular responses and the consequences in genome instability and oncogenesis. Today experimental approaches will be presented.

Mechanism of DNA replication in eukaryotes
Spatial and temporal regulation of replication
Telomere replication
Maintenance of telomere length; dysfunctions and senescence
Bypass of replication obstacles and damaged DNA
Interference with the transcriptional activity
Repair and signalling of DNA damages
Role of helicases in genome instability and pathologies
Impact of the chromosomal organization in bacterial rearrangements
Identification and mapping of genome rearrangements in tumour cells
Role of p53 mutations in human cancers and in mouse models.
Epidemiology and cancer predisposition in breast cancers
Targeting for cancer therapy

Single molecule visualization of an helicase activity by V. Croquette (ENS)