Cours international

Genetic Instability and Cancer - 2014

The aim of this course is to describe the current knowledges in the fields of DNA replication, repair and recombination and its consequences in genetic instability and cancer.

Key to the proper inheritance of the genetic information, cells should duplicate their genome before mitosis and avoid mutagenesis.

The course will describe the mechanisms and the regulation that ensures the maintenance of genetic information across cell generations. An emphasis will be put on:

initiation and completion of replication along the bulk chromosomes and at specific regions such as the telomeres and fragile sites; the pathways of DNA repair and recombination; the relationships linking replication stress to the formation of DNA damage; the signalling events surrounding intrinsic and environmentally induced DNA damage; the interference with transcriptional activity; the variety of cellular responses to damage including the generation of chromosome rearrangements and oncogenesis.

A large set of experimental methods involving genetic, molecular, cellular, and biochemical approaches will be taught.

Organizers : Alain Nicolas, Franck Toledo, Michelle Debatisse, Arturo Londono-Vallejo

Registration: click here

Master's 2 or PhD students from France and abroad.

A scientific article will be chosen in advance on the theme of the course, which will be assigned to M2 students. Students will present the article and discuss it orally along with their expanded bibliographical analysis in a 20 minute oral presentation, followed by a 15 minute discussion. This presentation can be evaluated by the scientific organizers for University credit.

All participants are required to attend the course in its entirety. Acceptance of PhD participants will require a signed letter from their thesis supervisor attesting the student’s availability for the whole week. This letter should be provided at the time of application, and should be submitted by Email to:

This course is validated by UPMC - Paris VI University. It provides 3 ECTS or an equivalent of 30 hours.

The courses will be in English. The participants and speakers will meet every day during lunch on site.

Selection of applicants will be made by the Course organization committee.

March 31 - April 4, 2014
15 January 2014

Amphithéâtre Pierre et Marie Curie
11 rue Pierre et Marie Curie
75005 Paris, France

January 20th, 2014

No registration fees are required

Alain Nicolas
Franck Toledo
Michelle Debatisse
Arturo Londono-Vallejo
Members of the Institut Curie Research Unit - CNRS UMR3244/UPMC

The aim is to provide an updated overview of the mechanisms and the regulation of DNA replication, repair, and recombination processes, the sources and the cell signalling processes of DNA damage, the cellular responses and the consequences of genome instability and oncogenesis. Experimental approaches will be presented.

- Mechanism of DNA replication in eukaryotes
- Spatial and temporal regulation of replication
- Replication of telomere and fragile sites
- Maintenance of telomere length, dysfunction, and senescence
- Bypass of replication obstacles and damaged DNA
- Role of helicases in genome instability and pathologies
- Identification and mapping of genome rearrangements in tumour cells
- Role of p53 mutations in human cancers and in mouse models
- Epidemiology and predisposition to breast cancers
- Targeting for cancer therapy

Single molecule visualization of helicase activity by V. Croquette (ENS), high throughput sequencing platform (Institut Curie).